誌上発表

誌上発表2008年（平成20年）

11．

T-2 toxin-induced toxicity in pregnant mice and rats

Doi, K. ^1),2), Ishigami, N .³⁾ and Sehata, S .⁴⁾
¹⁾ Nippon Institute for Biological Science
²⁾ Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
³⁾ Fukui Safety Research Laboratories, Ono Pharmaceutical Co., Ltd.
⁴⁾ Daiichi Sankyo Inc.
International Journal of Molecular Sciences, 9:2146-2158, 2008
Abstract: T-2 toxin is a cytotoxic secondary fungal metabolite that belongs to the trichothecene mycotoxin family. This mycotoxin is a well known inhibitor of protein synthesis through its high binding affinity to peptidyl transferase, which is an integral part of the ribosomal 60s subunit, and it also inhibits the synthesis of DNA and RNA, probably secondary to the inhibition of protein synthesis. In addition, T-2 toxin is said to induce apoptosis in many types of cells bearing high proliferating activity. T-2 toxin readily passes the placenta and is distributed to embryo/fetal tissues, which include many component cells bearing high proliferating activity. This paper reviews the reported data related to T-2 toxin-induced maternal and fetal toxicities in pregnant mice and rats. The mechanisms of T-2 toxin-induced apoptosis in maternal and fetal tissues are also discussed in this paper.

13．

NIBS系ミニブタの特性、生産体制と医学研究への応用

佐野順一
日本生物科学研究所
アニテックス、 20 ： 17 ‐ 22 、 2008
要旨： NIBS 系ミニブタは、一般財団法人日本生物科学研究所において確立した温順で白毛色の実験用ミニブタである。その起源は当所が 1967 年に米国より導入したピットマンムーア系に、さらにタイワン小耳種とゲッチンゲン系由来ミニブタの 3 種類のミニブタを交配し、小型、白毛色の NIBS 系ミニブタを作出した。このミニブタは主要組織適合性遺伝子座クラス Ⅱ領域の DRBI(D/D) 、 DQA(D/D) 、 DQB(SO9 あるいは S10/S10) 座位がホモに固定していることも判明した。飼育はコンベンショナル環境で行われており、輪番交配による生産方式を採用している。雌雄（ 6 ： 1 ）のミニブタで構成された群を 3 群設け、各群間の輪番交配により次世代の種動物群を構築している。 NIBS 系ミニブタは外科・移植分野、循環器分野、皮膚試験、薬理・代謝試験、試験、医療機器等の試験に広く活用されているが、さらなる医学分野への応用をめざして、 2004 年には体細胞クローンミニブタの作出を明治大学との共同研究によって成功させ、現在、ミニブタからヒトへの臓器移植を視野に入れた遺伝子改変ミニブタの作製に関する研究を行っている。 NIBS 系ミニブタは E 型肝炎ウイルス抗体およびサイトメガロウイルス抗体がフリーであることが判明しており、医学分野への応用がいっそう期待されている。

14．

mRNA Expression of Cytochrome P450 17αhydroxylase, Cytochrome P450 Aromatase, Anti-Mullerian Hormone, Estrogen Receptor α, and Androgen Receptor in Developing Gonads of Japanese Quail

Nakamura, K . ¹⁾ , Shibuya, K . ¹⁾ , Saito, N . ²⁾ , Shimada, K . ²⁾ ,
Ohshima, A . ¹⁾ , Hirai, T . ¹⁾ and Nunoya, T . ¹⁾
¹⁾ Nippon Institute for Biological Science
²⁾ Graduate School of Bioagricultural Sciences, Nagoya University
The Juournal of Poultry Science, 45: 298-302, 2008
Abstract: In the present study, we investigated mRNA expression of cytochrome P450 17αhydroxylase (P450c17), cytochrome P450 aromatase (P450arom), anti-Mullerian hormone (AMH), estrogen receptor α (ERα) and androgen receptor (AR) in the embryonic and post-hatching gonads of Japanese quail by using real-time RT-PCR. P450c17 mRNA expression in females was significantly higher than that in males at 16 days of incubation, 3 and 7 days after hatching. P450arom mRNA expression in females was markedly higher than that in males and there were significant differences at 3, 7 and 14 days after hatching. AMH mRNA expression in males was apparently higher than that in females and there were significant differences at 16 days of incubation and 3 days after hatching. ERα mRNA was highly expressed not only in females but also in males at 16 days of incubation. There were no meaningful trends of AR mRNA expressions in male and female gonads during the observation periods. It is likely that the profiles of mRNA expression of P450c17, P450arom, AMH, ERα and AR in the embryonic and post-hatching gonads of normal Japanese quail in the present study provide basic and useful information.

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